Phis2.1 载体图谱
Webb1 juni 2024 · Digest the pHIS2 vector with Sma I purify it by agarose gel electrophoresis (0.8% agarose). 5. For addition of a single ‘T’ base at the ends of the linearized pHIS2 plasmid, prepare the following mix in a 10 μL total volume: 1 μg pHIS2 ( Sma I linearized), 0.5 μL dTTP (stock concentration 10 mM), 1 μL 10× ExTaq buffer, 0.5 U ExTaq. WebbpHis2载体价格650元,pHis2载体质粒图谱(Vector map),载体序列(Sequence)见下文,载体质粒抗性为Kanamycin,质粒大小为7207bp。pHis2是酵母单杂交载体。
Phis2.1 载体图谱
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WebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog #K1617-1 Restriction Map and Multiple Cloning Site (MCS) of pHIS2 Vector. Unique restriction sites are in bold. *Note: The Sac II site is resistant to cleavage. Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding ... WebbpHIS2 can be maintained in both yeast and bacteria. It contains an autonomous replication se-quence ( ARS4) and TRP1 nutritional marker for replication and selection in yeast (1, …
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Webb1 sep. 2000 · The promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT. However, the colonies having both constructs (protein cloned in ...
Webb6 feb. 2024 · The chloroplast comprises the outer and inner membranes that are composed of the translocon protein complexes Toc and Tic (translocon at the outer/inner envelope membrane of chloroplasts), respectively. Tic55, a chloroplast Tic protein member, was shown to be not vital for functional protein import in Arabidopsis from previous … mingwinstallationmanagerWebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … mingw include pathWebbpHis2酵母单杂交载体是由Biovector Co.,LTD代理或销售的Biovector, Addgene, ATCC, Invi品牌的试剂,产品来源于Biovector Inc. USA。Biovector Co.,LTD是中国最权威的pHis2酵母单杂交载体试剂销售服务商之一,在海淀区等地方销售pHis2酵母单杂交载体试剂已经多年。生物在线为您提供众多企业pHis2酵母单杂交载体仪器产品及 ... mingw how to use sehWebbVector Database. Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene. Only the plasmids deposited at Addgene are available for purchase through this website. mingw include路径http://www.honorgene.com/product_list/carrier_library/123112.html most cherished possessionWebbpHISi is a yeast integration and reporter vector for use with the MATCHMAKER One-Hybrid System. pHISi contains the yeast HIS3 gene downstream of the MCS and the minimal … most chemically resistant stainless steelhttp://www.coolaber.com/usr/2160/File/F/F4671.pdf most cherished crossword clue