Human thp-1 monocytes
Although THP-1 cells are of the same lineage, mutations can cause differences as the progeny proliferates. In general, THP-1 cells exhibit a large, round, single-cell morphology. The cells were derived from the peripheral blood of a 1-year-old human male with acute monocytic leukemia. Some of their … Meer weergeven THP-1 is a human monocytic cell line derived from an acute monocytic leukemia patient. It is used to test leukemia cell lines in immunocytochemical analysis of protein-protein interactions, and Meer weergeven • Cellosaurus entry for THP-1 Meer weergeven WebStimulation of human monocyte THP-1 cells with LPS, BscF, and Tdap induced a robust elevation of CCL20, CXCL10, PGE2, and PGF2α among most chemokine and prostanoid members when compared with the ...
Human thp-1 monocytes
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WebTHP1-Blue™ were derived from the human THP-1 monocyte cell line by stable integration of an interferon regulatory factor (IRF)-inducible SEAP reporter construct. As a result, … WebFigure Legend Snippet: Cytotoxicity of the PaTx-II protein on cultured lung (MRC-5), skin fibroblasts (HEPK), and human macrophage (THP-1) cells incubated with 0.1–1000 µM …
Web14 mrt. 2008 · THP-1 monocytic cells have been extensively characterized, and they display phenotypic and functional features of mature human monocytes, including enhanced … Web18 nov. 2016 · THP-1 designates a spontaneously immortalized monocyte-like cell line, derived from the peripheral blood of a childhood case of acute monocytic leukemia (M5 …
Web16 jul. 2011 · Since this protein has important immune-modulatory properties, we have investigated the expression of Hsp60 in human THP-1 monocyte cells exposed to … WebHere we investigated phagocytic plasticity of THP-1 (acute monocytic human leukemia) cells at different stages (days 1, 3, and 7) of phorbol ester (PMA)-induced macrophage …
Websufficient quantities for experimentation and variability between donors. The human monocytic cell line THP-1 is widely used for the study of both monocytes and macrophages. THP-1 macrophages provide several advantages over tissue resident or peripheral blood mononuclear cell (PBMC) derived macrophages: ease of access, long …
WebTHP-1 cells are widely used for inflammasome studies due to their high expression levels of NLRP3, ASC, and pro-caspase-1. THP1-ASC-GFP cells are inflammasome reporter … secury guardWeb18 mei 2009 · Synergistic effect of fMLP and CCL2 on THP-1 cell and monocyte chemotaxis. (A) FACS analysis for FPR expression on THP-1 cells and peripheral blood-derived monocytes was performed as described in Materials and Methods. One experiment that is representative of two is shown. The solid curves represent the staining by the FPR … secu season ticketsWeb30 apr. 2024 · Activation of GPR30 with G1 inhibits oscillatory shear stress-induced adhesion of THP-1 monocytes to HAECs by increasing KLF2. Chi Chen, 1, * Jingyan … push factor definition human geographyWeb8 mrt. 2024 · The THP-1 cell line has been amply used for in vitro studies of monocyte-to-macrophage differentiation [ 34, 35] and as a model for macrophage polarization [ 36 – 38 ]. We adapted and optimized these protocols for macrophage differentiation and polarization into M1/M2 subsets. secusenWeb15 jun. 2024 · In this study, we investigated the ability of THP-1 monocytes and macrophages to accumulate lead (Pb) in vitro, relative to Pb concentration and length of … push factor for immigrantsWebThere are many different protocols for THP-1 differentiation to macrophages using PMA. 1) seed macrophages at a density of 1x106 cells/mL treated with 50 ng/mL of PMA. 3) after … push factor definition sociologyWeb26 jun. 2024 · Comparative transcriptomics of THP-1 monocytes in response to different pathogens exposed to different human pathogens, and evaluate its ability to develop … pushfactoren