Gapdh antibody dilution
WebMonoclonal Antibody for studying GAPDH. Cited in 5519 publications. Validated for WB, WB, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, GAPDH (D16H11) XP® Rabbit … WebGlyceraldehyde-3-phosphate dehydrogenase (GAPDH), a classic glycolytic enzyme, is a multi-functional protein. It is mainly present in the cytoplasm. GAPDH is ubiquitously …
Gapdh antibody dilution
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WebGAPDH antibody used for WB at 1:1000 dilution on lysates of mouse embryonal carcinoma P19 cell line which was induced to differentiate for 5 days while being … WebRabbit anti Human GAPDH (AHP1628) was used as the primary antibody at a dilution of 1/1000. Mouse anti Rabbit Light Chain:HRP MCA6003P was used as the secondary …
Web11 rows · Anti-GAPDH, Cat. No. AB2302, is a chicken polyclonal antibody that detects Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and is tested for use in Western … WebThe membrane was probed with a GAPDH polyclonal antibody (Product # PA1-987) at a dilution of 1:3000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room …
WebMy GAPDH is a rabbit polyclonal antibody, using dilution of 1:20000. But for secondary antibody, I used 1:5000. Sometimes I wonder would it be the cross-activity across the species.
WebMar 31, 2024 · The antibody dilutions were 800-fold in most cases except for GAPDH (5000-fold dilution), B-ACT and ALB (2000-fold dilution). Washing 5 times with T-TBS (200 µL/well). Incubation with secondary antibody 4000-fold diluted with blocker for 1 h at 37 °C with 400 rpm in the orbital shaker (100 µL/well).
WebAll lanes : Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603) at 1/50000 dilution Lane 1 : UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG, … tree slice clockWebPurified anti-GAPDH Antibody. Whole cell extracts (15 µg protein) from HeLa cells were resolved on a 4-12% Bis-Tris gel, transferred to a nitrocellulose membrane and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-GAPDH Antibody, clone FF26A/F9, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat ... trees landscaping ideasWebAll lanes : Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915) at 1/10000 dilution ... (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor ® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary ... trees laying outWebThe GAPDH (glyceraldehyde-3-phosphate dehydrogenase) gene is mapped to human chromosome 12p13.3 and encodes a tetramer containing identical chains. 1] The enzyme is commonly known as glycolytic enzyme and contains a binding site for NAD + (nicotinamide adenine diphosphate) and glyceraldehyde-3-phosphate. GAPDH contributes to 10-20% … trees lawn mowerWebGAPDH was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab181602 at 1/60 dilution. Western blot was … tree sleeping toscanaWebThere are 12 GAPDH antibodies which are validated in multiple tissues with various applications, including WB, IHC-P, ELISA, IP, ICC/IF. There are 9 GAPDH antibody for WB, 4 GAPDH antibody for IHC-P, 3 GAPDH antibody for ELISA, 4 GAPDH antibody for IP, 2 GAPDH antibody for ICC/IF. Among all these GAPDH antibodies, there are 3 anti … trees level crossinghttp://biopioneer.com.tw/?p=41026 trees leafing out early