WebSep 23, 2009 · FHCC98 and 7721 cells were harvested in a lysis buffer, and equal amounts of cellular proteins were subjected to SDS-PAGE (10%). Proteins were transferred to … WebFeb 6, 2013 · Human liver tumor-derived cell lines used in this study—including HepG2, Bel-7402, FHCC98, and Huh-7—were cultured under standard cell culture conditions in Dulbecco's modified Eagle's medium (DMEM) (GIBCO, Life Technologies) supplemented with 10% fetal bovine serum (GIBCO), 1% L -glutamine, 1% penicillin-streptomycin, and …
Immunoprecipitation of HAb18G/CD147 and integrin α6 and β1 …
WebDec 14, 2014 · Methods: The expression of Brachyury in human HCC (SMMC7721, HepG2, FHCC98, and Hep3B) and control cell lines was analyzed using quantitative reverse … WebMethods: The expression of Brachyury in human HCC (SMMC7721, HepG2, FHCC98, and Hep3B) and control cell lines was analyzed using quantitative reverse-transcriptase polymerase chain reaction and immunoflourence methods. Cancerous tissues collected from patients with HCC (n=112) were analyzed using immunohistochemical method; a gail\u0027s salads ocean city nj
Expression of C1orf61 in the HCC cell lines. A) C1orf61 …
WebApr 30, 2024 · Because USP21 is highly expressed in FHCC98 and HepG2 cells, these two cell lines were exploited for further study. shRNA-mediated inhibition of USP21 … WebOur previous study demonstrated that overexpression of HAb18G/CD147 promotes invasion by interacting with integrin α3β1. However, it has never been investigated whether α3β1 is solely responsible for this process or if other integrin family members also interact with HAb18G/ CD147 in human hepatoma cells. WebJul 13, 2024 · To investigate the role of miR-148a on tumor growth, an orthotopic liver cancer model was used. As shown in Supplementary Figure 2b and c, the tumors formed by miR-148a-3p sponge-expressing FHCC98 cells were bigger than those formed by control FHCC98 cells. In contrast, tumors formed by Pri-miR-148a expressing HepG2 cells were … gail\u0027s sandwiches