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WebDec 23, 2016 · Humans have over 400 copies of the gene encoding the 47S precursor rRNA (approximately 13 kb), which is processed to generate the mature 18S, 5.8S and 28S rRNAs, while the 5S rRNA is transcribed from a different locus [reviewed in 45,46]. A single 47S gene is separated from the next gene by a 30 kb intergenic spacer (IGS). WebAug 1, 2016 · Pork-specific primers for the LAMP assay were designed based on the mitochondrial D-loop regions, and eukaryotic primers based on the 18S rRNA gene were used for the endogenous control. The adoption of an endogenous control prevented false-negative results. The detection level was 1 pg for raw pork DNA and 0.1% of pork … gmea statewide honor chorus schedule
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