Cstps1

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August undefined, 2024

WebThe recombinant enzyme encoded by Cstps1 was shown to convert farnesyl diphosphate to a single sesquiterpene product identified as valencene by gas chromatography-mass … WebNov 18, 2003 · Alignment of Cstps1 with tobacco and cotton sesquiterpene synthase-deduced amino acid sequences.. Aligned sequences are of C. sinensis Cstps1 (this …

Valencene synthase - Trends in Plant Science

WebOct 19, 2024 · CsTPS1-3 have predicted chloroplast-target peptides and produced predominantly linalool when GPP was supplied as substrate. Furthermore, CsTPS9 catalysed the formation of ( E )-β-ocimene and myrcene, and small amounts of sabinene and ( Z )-β-ocimene from GPP. WebSep 1, 2011 · Expression of a cytosolic form of CsTPS1 in addition to mtCsTPS1 further elevated valencene production levels by approximately 25%, an amount (300 μg/l) that … flower kittle architects

Functional Expression and Characterization of Trichome-Specific ...

CsTPS1 has previously been characterized as the key gene encoding the enzyme terpene synthase that catalyzes (+)-valencene biosynthesis (Sharon-Asa et al., 2003). Therefore, we screened a large set of citrus transcription factors for activation or inhibition using the promoter of CsTPS1 and a dual-luciferase assay ( Supplementary Fig. S1 ). WebTwo recombinant, stereospecific monoterpene synthases, a (-)-limonene synthase (CsTPS1) and a (+)-α-pinene synthase (CsTPS2), encoded by Cannabis sativa L. cv. ‘Skunk’ trichome mRNA, have been isolated and characterized. Recombinant CsTPS1 showed a Km value of 6.8 μM, a Vmax of 1.1 x 10-4 µmol/min and V WebJun 1, 2004 · The characterization of the Cstps1 gene now provides researchers with an opportunity to engineer plants for high-level production of valencene, a highly valued flavor and fragrance compound. Adapted … green acres lawn care fl

Citrus fruit flavor and aroma biosynthesis: isolation, functional ...

WebCsTPS1 encodes for a monoterpene synthase that contributes to the emission of a blend of volatile compounds emitted from flowers of Camelina sativa. The work describes the in vitro characterization of a monoterpene synthase and its regulatory region that we cloned from Camelina sativa (Camelina). Here, we named this gene as C. sativa terpene synthase 1 … WebJul 28, 2012 · The Cstps1 expression was detected in different organs, with high expression levels in leaf and flower, weak expression in stem and root. Real-time qRT-PCR … flower kits for flowersWebThe recombinant enzyme encoded by Cstps1 was shown to convert farnesyl diphosphate to a single sesquiterpene product identified as valencene by gas chromatography-mass spectrometry (GC-MS). Phylogenetic analysis of plant terpene synthase genes localized Cstps1 to the group of angiosperm sesquiterpene synthases. flower kits for weddings

"WebMar 1, 2007 · Recombinant CsTPS1 showed a K m value of 6.8 μM, a V max of 1.1 × 10 −4 μmol/min and V max /K m of 0.016; the pH optimum was determined at pH 6.5, and a temperature optimum at 40°C. Recombinant CsTPS2 showed a K m value of 10.5 μM, ... " - Cstps1

Cstps1

Characterization of˜a˜monoterpene synthase gene from

WebDec 5, 2013 · • Citrus volatiles confer protection against endoparasitoid wasps Summary Background Egg-laying animals, such as insects, ensure the survival of their offspring by depositing their eggs in favorable environments. To identify suitable oviposition sites, insects, such as the vinegar fly Drosophila melanogaster, assess a complex range of … WebSep 30, 2024 · Nimbus Therapeutics Publishes Structural Analysis Highlighting Mechanisms for Selective Inhibition of CTPS1. CAMBRIDGE, Mass. – September 30, 2024 – Nimbus …

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WebDec 1, 2003 · Cstps1 was found to be developmentally regulated: transcript was found to accumulate only towards fruit maturation, corresponding well with the timing of valencene accumulation in fruit. Although citrus fruits are non‐climacteric, valencene accumulation and Cstps1 expression were found to be responsive to ethylene, providing further evidence ... WebJul 8, 2024 · The proteins encoded by the full-length terpene synthase ( CsTPS1 ) and its alternative splicing isoform ( CsTPS1 - AS ) could catalyze the formation of geraniol when GPP was used as a substrate in vitro , whereas the expression of CsTPS1 - AS was only significantly induced by Colletotrichum gloeosporioides and Neopestalotiopsis sp. infection.

WebNov 18, 2003 · Alignment of Cstps1 with tobacco and cotton sesquiterpene synthase‐deduced amino acid sequences.. Aligned sequences are of C. sinensis Cstps1 (this work), Nicotiana tabacum epi‐aristolochene synthase (Accession # T03714), and Gossypium arboreum cadinene synthase (Accession # CAA77191).Conserved sequence … WebBiochemical goals • In Citrus sinensis, the Cstps-1 gene codes for the production of the enzyme Citrus Valancene Synthase (CVS). • In vivo, CVS reacts with farnesyl pyrophosphate (FPP) to produce the characteristically fragrant valencene (as well as 5 …

WebMar 13, 2024 · Results. There was a clear relationship between presence or absence of the valencene synthase gene (Cstps1) expression, and presence or absence of valencene among randomly selected mandarin hybrids.Cloning the coding regions of Cstps1 from Fortune and Murcott mandarin, and aligning with previous reported Valencia orange … WebNon-small cell lung carcinoma (NSCLC) cells with oncogenic KRAS and loss of the tumor suppressor LKB1 express CPS1 and depend on this enzyme for growth. CPS1 maintains …

Webthat CitAP2.10 could trans-activate the CsTPS1 promoter. Ethylene enhanced expression of CitAP2.10 and this effect was abolished by the ethylene antagonist 1 …

WebNov 18, 2003 · Alignment of Cstps1 with tobacco and cotton sesquiterpene synthase‐deduced amino acid sequences.. Aligned sequences are of C. sinensis Cstps1 … flower king fireworksWebApr 30, 2024 · Our results uncovered seven CsTPS genes in the cucumber genome and named CsTPS1-CsTPS7 according to their locations in the chromosomes. Seven CsTPS genes were randomly distributed in six cucumber chromosomes. flower knight equipment upgradeWebMay 18, 2016 · The expression pattern of CitAP2.10 was positively correlated with (+)-valencene content and CsTPS1 expression. Dual-luciferase assays indicated that … flower kittyWebCTP synthase 1. CTP synthase 1 is an enzyme that is encoded by the CTPS1 gene in humans. [5] [6] CTP synthase 1 is an enzyme in the de novo pyrimidine synthesis … green acres lawn care paWebMar 6, 2015 · An enzyme encoding valencene synthase gene (Cstps1) was more abundant in Temple than in Murcott. Valencene accounted for 9.4% of total volatile content in Temple, whereas no valencene was detected in Murcott fruit. Murcott expression of Cstps1 is severely reduced. Conclusion: flower knight girl 5chWebCsTPS1- AS reduced the accumulation of geraniol, and the silenced tea plants exhibited greater susceptibility to pathogen infection than control plants. However, the geraniol content and pathogen resistance of CsTPS1-silenced plants and control plants did not signi cantly di er. Further analysis showed that silencing of CsTPS1- AS led to a ... flower kitty gifWebJan 7, 2016 · Escherichia coli (pRSF-CsTps1 and pAC-Mev/Scidi/Aacl) was similarly cultured, extracted, and analyzed by GC–MS. As a result, only one peak generated, which was determined to be linalool by the MS … flower_knight_girl