WebFeb 8, 2024 · The expression of circANKS1B and miR‐152‐3p was analyzed by real‐time quantitative reverse‐transcription polymerase chain reaction (qRT‐PCR). Cell migration and invasion were measured using a transwell assay. The interaction between circANKS1B and miR‐152‐3p was confirmed by a dual‐luciferase reporter gene assay. WebBackground: A growing number of studies indicate that circular RNAs (circRNAs) play critical roles in human diseases, and show great potential as biomarkers and therapeutic …
Fig. 1 Identification of circular RNAs by RNA-seq and the...
We characterized circRNA transcripts by performing RNA-seq on ribosomal RNA-depleted total RNA from three pairs of TNBC and adjacent normal tissues. In all, 69,815 distinct circRNAs were found in this study and 87% were derived from exons, and the others were derived from introns, intergenic region and 3′ … See more To dissect the underlying mechanism by which circANKS1B induces EMT to promote breast cancer metastasis, we mainly focus on “miRNA sponges” because of … See more USF1, a transcription factor, belongs to the basic helix-loop-helix leucine zipper (bHLH-LZ) family which regulates different genes expression by … See more WebMethods: CircANKS1B (a circRNA originated from exons 5 to 8 of the ANKS1B gene, hsa_circ_0007294) was identified by RNA-sequencing and validated by qRT-PCR and … raymond james hall of fame classic
circRNAs and their relationship with breast cancer: a review
WebDec 3, 2024 · Consequently, circANKS1B induces EMT by sponging miR-148a-3p and miR-152-3p. 83 Moreover, upregulated circANKS1B expression, which promotes migration and invasion of cancer cells, served as an independent risk factor for poor OS in patients with BC. 83 Other circRNAs, such as circAGFG1, circKIF4A, and circPLK1, are also … http://jcmp.yzu.edu.cn/ WebFeb 11, 2024 · Similarly, enrichment of circANKS1B in HPV-positive cervical cancer exosomes may play a role in influencing cellular migration and invasion. circANKS1B increases the expression of transcription factor USF1, which upregulates TGF-β1 expression resulting in activated TGF-β1/Smad signaling and EMT in breast cancer . simplic rainmeter