WebNov 19, 2024 · 小鼠小胶质BV2传代方法:. 1、轻轻吸去培养上清液,留下2-3ml培养基,轻轻吹打细胞表面,吹掉细胞;. 2、混匀细胞,收集细胞培养基,900rpm离心3-5分钟,弃上清;. 3、加入新的*培养基,轻轻重悬细胞,均匀分布到新的培养瓶中;. 4、补充完整培养基,将培养瓶 ... WebMar 22, 2012 · 小胶质细胞 BV2作为中枢神经系统内的免疫细胞,既可通过吞噬脑组织中的病原体及有害颗粒对神经元起保护作用,又可在致炎因子的作用下,激活成反应性小胶 …
BV2 (小鼠小胶质细胞) (STR鉴定正确) - 武汉普诺赛生命科技 ...
WebDec 19, 2014 · 综上所述,1 10 -8 mol 鱼藤酮明显促进BV2细胞生成一氧化氮而不影响细胞存活率;当鱼藤酮 10-7 mol /L时可使细胞存活率明显降低。. 一点说明鱼藤酮对小胶质细胞的激活效应可在一定的浓度范围内存在,超出该范围时则对小胶质细胞 可造成损伤乃至死 … WebSep 17, 2024 · Protocol for checking the role of ROS in generation of traps and the functionality of traps will take 4–5 days from seeding of the BV2 cells. MTT assay will take 3 days from the seeding of cells. This step will determine whether the extracellular traps formation is dependent or independent of cell death, reactive oxygen species (ROS) and ... havilah ravula
小胶质细胞亚型和标志物概述 Abcam中文官网
WebMay 20, 2024 · 注意事项. 1. 该细胞对血清质量要求高,推荐使用HAKATA优等胎牛血清。. 2. 该细胞对外界刺激较为敏感,建议使用新鲜的DMEM高糖培养基,培养基出现发紫现象时不再使用,且及时更换新鲜培养基。. 3. 该细胞贴壁不牢,会有部分细胞悬浮。. 换液时勿用PBS进行冲洗 ... http://www.whprocell.com/Products-35920110.html WebBV2 microglia were treated with different concentrations of LPS for 24 h. Western blot was utilized to detect the expression of TLR4 and the downstream signaling pathway. The level of inflammatory mediator was quantified using a specific ELISA kit. The supernatant of 10 μg/ml LPS-treated BV2 cells was used as conditioned medium (CM). havilah seguros